The study sought to assess the chemical composition, microbial quality and in vitro gas production of rice straw supplemented with pelleted or unpelleted dried rumen digesta (DRD) concentrate in the savannah agro-ecological zone of Ghana. Four levels of DRD (0%, 5%, 10% and 15%) were incorporated into a concentrate and subjected to two methods of processing (unpelleted diet and pelleted diet). Processing methods and DRD inclusion levels had a significant (P<0.05) interaction effect on crude protein (CP), ash, lactic acid bacteria (LAB) and E. coli. The unpelleted diet at a 15% inclusion level of dried DRD had the highest CP (14.22%).