These guidelines focus on responsible use of antimicrobials in sustainable apiculture. Following a one-health approach, they aim to protect not only honey bees, but even human health (e.g. reducing the risks of residues in hive products and preventing development of antimicrobial resistance) and the environment. The best way to reach this goal is to prevent and to guarantee the early detection of clinical cases of the main honey bee diseases through the application of good beekeeping practices and biosecurity measures.