Plant response to stress is critical and can be harnessed for greater yield increases in productive agriculture. Agriculture is susceptible to climate variability which impacts largely on food security by changing the balance among the key determinants of crops and yield. Enormous genetic diversity for stress tolerance such as Heat Shock Protein (HSP) genes exist in this plant species. Recent advances in sequencing technologies have rapidly unfolded the molecular genetic basis for heat stress responses in plants. In this regard, the role of heat-responsive HSP70 genes in Sorghum bicolor is investigated. The genome sequence of S. bicolor, retrieved from Heat Shock Protein Information Resource (HSPIR), database was studied using bioinformatics tools. Sequence analysis revealed 11 and 12 pairs of paralogous, for HSP70, indicating ancestral duplication and the crucial role in the expansion of S.